The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental to investigations of cell cycle–regulated protein expression ...

Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...

LSC complements flow cytometry, allowing analysis of samples ... make it a preferred choice for studies requiring simple or multiplexed cell cycle analysis of adherent cells.

One important aspect of DNA analysis is the ability of the cytometer to exclude cell doublets. In a cytometer where the beam diameter is larger than the nuclear size, we can look at pulse width versus ...