This work introduces an important new method for depleting ribosomal RNA from bacterial single-cell RNA sequencing libraries, demonstrating its applicability for studying heterogeneity in microbial ...

Methods We analysed bulk RNA-seq data for enrichment of biological processes, transcription factors (TFs) and deconvolution-based immune cell types from peripheral blood mononuclear cells (PBMCs) in ...

Computational biologists from the National University of Singapore (NUS) have uncovered how RNA splicing—a crucial process ...

Antibody cloning and expression from single B cells is then required for functional assays. Here we propose a method that integrates FACS-based 5’-end single-cell RNA sequencing (FB5P-seq) and ...

Then, scientists can analyze the composition of RNA molecules that contain codes for building new proteins. "However, typical single-cell sequencing experiments use what is called short-read ...

Methods: Four microarray datasets and a high throughput sequencing dataset were acquired from GEO database to reveal the PGK1 expression in patients of sepsis. Quantitative real-time PCR and western ...

This heterogeneous nature has hampered a better understanding of SLE pathogenesis and the development of effective therapeutic agents. While recent single-cell RNA sequencing studies of SLE identified ...

This important discovery helps explain how individuals' genetic differences contribute to their predisposition to complex diseases such as systemic lupus erythematosus (SLE) and Graves' disease (GD).